کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1950503 | 1055644 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nuclear envelope proteins Nesprin2 and LaminA regulate proliferation and apoptosis of vascular endothelial cells in response to shear stress
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کلمات کلیدی
Activator protein-2TBP-associated factorsONMTAFsLINCINMNSSAP-2IPAHRPTBPECs - EC هاIfng - ifngsmall interfering RNAs - RNA های تداخل کوچکsiRNA - siRNADysfunction - اختلال عملکردTem - این استinterferon-gamma - اینترفرون گاماVascular remodeling - بازسازی عروقIngenuity Pathway Analysis - تجزیه و تحلیل راه IngenuityShear stress - تنش برشیlow shear stress - تنش برشی کمEndothelial cells - سلولهای اندوتلیالTranscription factor - عامل رونویسیTranscription factors - عوامل رونویسیOuter nuclear membrane - غشای هسته بیرونیInner nuclear membrane - غشای هسته داخلیlinker of nucleoskeleton and cytoskeleton - لینک دهنده هسته سلول و سی تی اسکنTransmission electron microscope - میکروسکوپ الکترونی انتقالnuclear envelope - پاکت هسته ایPET - پتHorseradish peroxidase - پراکسیداز هوررادیشTATA-box binding protein - پروتئین اتصال TATA جعبهPolyethylene terephthalate - پلی اتیلن ترفتالاتnegative control - کنترل منفی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The dysfunction of vascular endothelial cells (ECs) influenced by flow shear stress is crucial for vascular remodeling. However, the roles of nuclear envelope (NE) proteins in shear stress-induced EC dysfunction are still unknown. Our results indicated that, compared with normal shear stress (NSS), low shear stress (LowSS) suppressed the expression of two types of NE proteins, Nesprin2 and LaminA, and increased the proliferation and apoptosis of ECs. Targeted small interfering RNA (siRNA) and gene overexpression plasmid transfection revealed that Nesprin2 and LaminA participate in the regulation of EC proliferation and apoptosis. A protein/DNA array was further used to detect the activation of transcription factors in ECs following transfection with target siRNAs and overexpression plasmids. The regulation of AP-2 and TFIID mediated by Nesprin2 and the activation of Stat-1, Stat-3, Stat-5 and Stat-6 by LaminA were verified under shear stress. Furthermore, using Ingenuity Pathway Analysis software and real-time RT-PCR, the effects of Nesprin2 or LaminA on the downstream target genes of AP-2, TFIID, and Stat-1, Stat-3, Stat-5 and Stat-6, respectively, were investigated under LowSS. Our study has revealed that NE proteins are novel mechano-sensitive molecules in ECs. LowSS suppresses the expression of Nesprin2 and LaminA, which may subsequently modulate the activation of important transcription factors and eventually lead to EC dysfunction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 5, May 2015, Pages 1165-1173
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1853, Issue 5, May 2015, Pages 1165-1173
نویسندگان
Yue Han, Lu Wang, Qing-Ping Yao, Ping Zhang, Bo Liu, Guo-Liang Wang, Bao-Rong Shen, Binbin Cheng, Yingxiao Wang, Zong-Lai Jiang, Ying-Xin Qi,