کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1951983 1538415 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel antifungal peptide from leaves of the weed Stellaria media L
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A novel antifungal peptide from leaves of the weed Stellaria media L
چکیده انگلیسی


• A novel peptide named SmAMP3 was isolated from leaves of common chickweed (Stellaria media L.).
• SmAMP3 belongs to the family of hevein-like AMPs carrying a conserved chitin-binding site.
• The peptide exhibits potent antifungal activity against plant pathogens in the micromolar range.
• This peptide is constitutively expressed in above-ground organs and seeds of S. media plants.

A novel peptide named SmAMP3 was isolated from leaves of common chickweed (Stellaria media L.) by a combination of acidic extraction and a single-step reversed-phase HPLC and sequenced. The peptide is basic and cysteine-rich, consists of 35 amino acids, and contains three disulphide bridges. Homology search revealed that SmAMP3 belongs to the family of hevein-like antimicrobial peptides carrying a conserved chitin-binding site. Efficient binding of chitin by SmAMP3 was proved by in vitro assays. Molecular modeling confirmed conservation of the chitin-binding module in SmAMP3 locating the variable amino acid residues to the solvent-exposed loops of the molecule. The peptide exhibits potent antifungal activity against important plant pathogens in the micromolar range, although it is devoid of antibacterial activity at concentrations below 10 μM. As judged by chromatographic behavior and mass spectrometric data, the peptide is constitutively expressed in above-ground organs and seeds of S. media plants, thus representing an important player in the preformed branch of the plant immune system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 116, September 2015, Pages 125–132
نویسندگان
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