کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1952616 1057218 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Integrin signaling and cell spreading alterations by rottlerin treatment of chick limb bud mesenchymal cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Integrin signaling and cell spreading alterations by rottlerin treatment of chick limb bud mesenchymal cells
چکیده انگلیسی

Endochondral skeletal development begins with the formation of a cartilaginous template where mesenchymal cells aggregate and increase in density prior to their overt differentiation into chondrocytes. Prechondrogenic condensation, in which mesenchymal cells aggregate, requires cell migration and proliferation. However, the molecular mechanisms promoting this aggregation remain to be elucidated. Here, we report that rottlerin suppresses migration and cell surface expression of integrin β1 in chondrogenic progenitors. Perturbation of integrin β1 function using an anti-integrin β1 blocking antibody suppressed the migration of wing bud mesenchymal cells. Furthermore, phosphorylation levels of Src and focal adhesion kinase (FAK) were decreased by rottlerin treatment. Cell treatment with PP2, an inhibitor of Src family kinase, or electroporation of FAK specific siRNA, suppressed cell migration in a wound-healing assay. Cells treated with rottlerin showed decreased phosphorylation of Akt, independent of PKCδ inhibition. In addition, an Akt inhibitor suppressed the migration of chick limb bud mesenchymal cells. Taken together, our results point to the novel finding that rottlerin may act as a negative regulator for cell migration, an essential step for prechondrogenic condensation, by regulating integrin β1 signaling at focal adhesion complexes via modulation of Akt activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 91, Issue 5, May 2009, Pages 624–631
نویسندگان
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