کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1953184 1057255 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Class-1 release factor eRF1 promotes GTP binding by class-2 release factor eRF3
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Class-1 release factor eRF1 promotes GTP binding by class-2 release factor eRF3
چکیده انگلیسی

In eukaryotes, termination of mRNA translation is triggered by the essential polypeptide chain release factors eRF1, recognizing all three stop codons, and eRF3, a member of the GTPase superfamily with a role that has remained opaque. We have studied the kinetic and thermodynamic parameters of the interactions between eRF3 and GTP, GDP and the non-hydrolysable GTP analogue GDPNP in the presence (KD(GDP) = 1.3 ± 0.2 μM, KD(GTP) ≈ 200 μM and KD(GDPNP) > 160 μM) as well as absence (KD(GDP) = 1.9 ± 0.3 μM, KD(GTP) 0.7 ± 0.2 μM and KD(GDPNP) ≈ 200 μM) of eRF1. From the present data we propose that (i) free eRF3 has a strong preference to bind GDP compared to GTP (ii) eRF3 in complex with eRF1 has much stronger affinity to GTP than free eRF3 (iii) eRF3 in complex with PABP has weak affinity to GTP (iv) eRF3 in complex with eRF1 does not have strong affinity to GDPNP, implying that GDPNP is a poor analogue of GTP for eRF3 binding.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 88, Issue 7, July 2006, Pages 747–757
نویسندگان
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