کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1953509 1057282 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Probing the folding capacity and residual structures in 1–79 residues fragment of staphylococcal nuclease by biophysical and NMR methods
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Probing the folding capacity and residual structures in 1–79 residues fragment of staphylococcal nuclease by biophysical and NMR methods
چکیده انگلیسی

1–79 residues SNase fragment (SNase79) has chain length containing a sequence for helix α1, ω-loop, βI-sheet, and partial βII-sheet of native SNase. The incomplete “β-barrel” structural region of SNase79 makes this fragment to be interested in investigation of its conformation. For this study, we use CD, fluorescence, and NMR spectroscopy to probe the folding capacity and the residual structures in SNase79. The optical spectra obtained for SNase79 and its mutants reveal the presence of retained capacity for folding of the fragment. The NMR derived 13Cα secondary chemical shifts, 3JNH–Hα coupling constants, amide-proton temperature coefficients, interresidue NOEs, and 15N relaxation data determine the intrinsic propensities for helix- and turn- or β-sheet-like conformations of SNase79, which is not the result of stabilizing inter-molecular interactions by oligomerization effects. The residual turn- and helix-like structures may serve as potential local nucleation sites, whereas the residual βI-sheet-like structure can be regarded as a potential non-local nucleation site in the folding of SNase79. The intrinsic local and non-local interactions in these potential initiation sites are insufficient to stabilize the folding of SNase79 due to the shortage of relevant long-range interactions from other part of the fragment. The conformational ensemble of SNase79 is a highly heterogeneous collection of interconverting conformations having transiently populated helix- and β-sheet- or turn-like structures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 88, Issue 10, October 2006, Pages 1343–1355
نویسندگان
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