کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1953534 1538438 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interplay between the cis-prenyltransferases and polyprenol reductase in the yeast Saccharomyces cerevisiae
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Interplay between the cis-prenyltransferases and polyprenol reductase in the yeast Saccharomyces cerevisiae
چکیده انگلیسی

Dolichol formation is examined in three Saccharomyces cerevisiae strains with mutations in the ERG20 gene encoding farnesyl diphosphate synthase (mevalonic acid pathway) and/or the ERG9 gene encoding squalene synthase (sterol synthesis pathway) differing in the amount and chain length of the polyisoprenoids synthesized. Our results suggest that the activities of two yeast cis-prenyltransferases Rer2p and Srt1p and polyprenol reductase are not co-regulated and that reductase may be the rate-limiting enzyme in dolichol synthesis if the amount of polyisoprenoids synthesized exceeds a certain level. We demonstrate that reductase preferentially acts on typical polyprenols with 13–18 isoprene residues but can reduce much longer polyprenols with even 32 isoprene residues.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 88, Issues 3–4, March–April 2006, Pages 271–276
نویسندگان
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