کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1963995 | 1058521 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protein kinase CK2 interacts with adiponectin receptor 1 and participates in adiponectin signaling
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کلمات کلیدی
dmaTdithiobis[succinimidylpropionate]CK2βAdipoR1IRS1AdipoRAMPKGADDSPACCadiponectin - آدیپونکتینglobular adiponectin - آدیپونکتین کرویacetyl-CoA carboxylase - استیل کروکسی سیلازinsulin receptor substrate - انسولین بستر گیرندهFAD - بدyeast two-hybrid - مخمر دو رقمیProtein kinase CK2 - پروتئین کیناز CK2adiponectin receptor - گیرنده adiponectin
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Protein kinase CK2 interacts with adiponectin receptor 1 and participates in adiponectin signaling Protein kinase CK2 interacts with adiponectin receptor 1 and participates in adiponectin signaling](/preview/png/1963995.png)
چکیده انگلیسی
Adiponectin is an adipokine with anti-atherogenic, anti-diabetic and insulin sensitizing properties. Its effects on energy homeostasis, glucose and lipid metabolism are mediated by two ubiquitously expressed seven-transmembrane receptors, AdipoR1 and -R2. With the exception of APPL1 and RACK1, no intracellular binding partners of adiponectin receptors are reported and thus signaling pathways downstream of these receptors remain largely unknown. To incorporate adiponectins protective potential in drug development it is essential to understand adiponectin signaling cascades in detail. A yeast two-hybrid approach employing AdipoR1s cytoplasmatic N-terminus led to the identification of the regulatory subunit of protein kinase CK2. We confirmed the interaction in co-immunoprecipitation, ELISA experiments and co-localization analysis in mammalian cells. Furthermore we could localize the interaction site in an N-terminal basic region close to the transmembrane domain. In adiponectin stimulation experiments of C2C12 mouse myotubes and MCF7 cells incorporating CK2 inhibitor 2-dimethylamino-4,5,6,7-tetrabromo-1H-benz-imidazole (DMAT) we found a modulator role of CK2 in adiponectin signaling. Accordingly we identified the regulatory subunit of protein kinase CK2 as a novel intracellular partner of AdipoR1 and have strong evidence of CK2 as an effector molecule in adiponectin signaling. Since CK2 is involved in signaling cascades of other adipokines and hormones, e.g. leptin and insulin, our findings suggest a possible key function in crosstalk between adiponectin and insulin signaling pathways and could provide further insight into the anti-diabetic effects of adiponectin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 21, Issue 6, June 2009, Pages 936-942
Journal: Cellular Signalling - Volume 21, Issue 6, June 2009, Pages 936-942
نویسندگان
John T. Heiker, Cornelia M. Wottawah, Cathleen Juhl, David Kosel, Karin Mörl, Annette G. Beck-Sickinger,