کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1966063 | 1538693 | 2012 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Fast and simple LC–MS/MS method for quantifying plasma voriconazole Fast and simple LC–MS/MS method for quantifying plasma voriconazole](/preview/png/1966063.png)
BackgroundAn increasing number of publications point to the importance of voriconazole plasma monitoring. Our aim was to develop a fast and simple LC–MS/MS method for the quantification of plasma voriconazole.Methods20 μL of plasma was extracted by adding a precipitation reagent containing the internal standard (d3-voriconazole). Analysis was performed on a Quattro Micro tandem mass spectrometer equipped with an Alliance HPLC 2795 separations module. MRM transitions were m/z 350.0→281.4 for voriconazole and m/z 353.0→284.4 for d3-voriconazole. Quantification was done by both linear calibration curves and multiplication of the response ratio by a predefined factor.ResultsA method using protein precipitation as only pretreatment with an analysis time of 2 min was developed. Within- and between-run precision, expressed as coefficient of variation, at 6 concentrations ranged from 2.8% to 11.7%. Accuracy, expressed as a percentage of the theoretically added concentration, ranged from 89.0% to 109.0%. Linearity was demonstrated from 0.06 mg/L (0.17 μM) to 20.0 mg/L (57.3 μM).ConclusionsThe described method offers a simple and rapid analysis of voriconazole in human plasma for clinical routine. Quantification with a predefined factor permits omitting calibration to each run and thereby reduces workload, costs and turn-around time.
Journal: Clinica Chimica Acta - Volume 413, Issues 7–8, 11 April 2012, Pages 740–743