No association between common Gly972Arg variant of the insulin receptor substrate-1 and polycystic ovary syndrome in Southern Chilean women

BackgroundPreviously studies have indicated that the insulin receptor substrate-1 (IRS-1) Gly972Arg (G972R) polymorphism is associated with polycystic ovary syndrome (PCOS). We examined the possible association between G972R common variant of the IRS-1 gene and PCOS in Southern Chilean women with PCOS and controls.MethodsA total of 50 women with PCOS (29.1 ± 8.1 yr) and 75 healthy women (29.3 ± 9.3 yr) were included. Serum lipids, glucose and uric acid concentrations were determined by enzymatic-colorimetric methods. The G972R variant of the IRS-1 gene was detected by PCR–RFLP.ResultsWomen with PCOS exhibited a higher concentrations of total testosterone, glucose, insulin, total cholesterol, triglycerides, LDL-C and uric acid, and lower HDL-C concentrations than controls (P < 0.05). The presence of G972R polymorphism in PCOS and control women was not significantly different (16% vs. 6.6%, P = 0.276). The OR for PCOS associated to 972R variant was 2.67 (95% CI: 0.82–8.69, P = NS). Moreover, neither association between G972R genotypes and metabolic parameters were observed in PCOS women or controls.ConclusionOur data do not support an association between G972R variant of the IRS-1 with PCOS or its metabolic parameters in Southern Chilean women.