Voltammetric behavior and determination of estrogens at carbamylcholine modified paraffin-impregnated graphite electrode

Carbamylcholine (CCh) modified paraffin-impregnated graphite electrode (CCh/WGE) was prepared and characterized by using X-ray photoelectron spectroscopy, UV–vis spetroelectrochemistry and cyclic voltammetry. It was found that the CCh/WGE could be used for surface accumulation and electrocatalytic determination of estrogens involving estradiol, estrone and estriol. By using square-wave stripping voltammetry, an anodic peak was observed at about 0.55 V versus SCE for all these estrogens. The peak current was proportional to concentration of estradiol, estrone and estriol in the range from 4 × 10−6 to 4 × 10−5, 3 × 10−7 to 3 × 10−5, and 5 × 10−7 to 9 × 10−6 M, respectively. This electrode could be used for determination of estrogens in practical samples.