کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1967472 | 1538748 | 2007 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Clinical significance of ELISA positive and immunofluorescence negative anti-dsDNA antibody Clinical significance of ELISA positive and immunofluorescence negative anti-dsDNA antibody](/preview/png/1967472.png)
BackgroundPositivity for anti-dsDNA antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). In the present study, the significance of ELISA positive and Crithidia luciliae immunofluorescence test (CLIFT) negative anti-dsDNA sera was evaluated.MethodsThere were 371 consecutive serum samples submitted to for anti-dsDNA testing that were assayed using anti-dsDNA ELISA and CLIFT. Sera showing discrepant results were collected and then examined using 3 commercial anti-dsDNA and anti-ssDNA ELISA kits and by Farr assay. Medical records were reviewed for those patients who were ELISA positive and CLIFT negative for anti-dsDNA.ResultsFifty-two patients of 100 anti-dsDNA ELISA positive patients were negative by CLIFT. For ELISA positive and CLIFT negative sera, Farr assays showed the highest positive rate (72.7%) for the 4 different anti-dsDNA assays (3 commercial kits and the Farr assay). Nearly 80% of 44 ELISA positive and CLIFT negative patients met ≥ 3 of the SLE classification criteria (excluding the anti-dsDNA criterion).ConclusionSome anti-dsDNA ELISA kits have diagnostic efficiencies that are similar to that of the Farr assay. Moreover, the study identifies a group of patients that are ELISA positive but CLIFT negative for anti-dsDNA, and indicates that the majority of these patients have clinically relevant SLE.
Journal: Clinica Chimica Acta - Volume 380, Issues 1–2, 1 May 2007, Pages 182–185