کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
196776 | 459852 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Hot electron-induced cathodic electrochemiluminescence of rhodamine B at disposable oxide-coated aluminum electrodes Hot electron-induced cathodic electrochemiluminescence of rhodamine B at disposable oxide-coated aluminum electrodes](/preview/png/196776.png)
Rhodamine B (RhB) exhibits strong cathodic electrogenerated chemiluminescence (ECL) in aqueous solutions during high-amplitude pulse polarization at thin oxide film-coated aluminum electrodes. This method allows the detection of RhB below nanomolar concentration level and provides linear calibration plots spanning over several orders of magnitude of concentration. In addition, a relatively long ECL lifetime of RhB provides a basis for time-resolved detection. Thus, widely used RhB-based labels can also be suggested to be usable as electrochemiluminescent labels in fully aqueous solutions in bioaffinity assays such as in immunoassays and DNA-probing assays. Support was obtained for the chemiluminescence generation mechanism to be essentially the same as that of radiochemiluminescence in aqueous solution.
Journal: Electrochimica Acta - Volume 51, Issue 13, 5 March 2006, Pages 2706–2714