کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1968167 1538761 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Catecholamine analysis with microcolumn LC-peroxyoxalate chemiluminescence reaction detection
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Catecholamine analysis with microcolumn LC-peroxyoxalate chemiluminescence reaction detection
چکیده انگلیسی

BackgroundPlasma catecholamines (CAs) are widely used as an index of sympathetic nervous system activity. In addition, CAs are known to be metabolized by catechol-O-methyltransferase (COMT) to produce their 3-O-methyl metabolites. We previously established a sensitive determination method of CAs and their 3-O-methyl metabolites using HPLC-peroxyoxalate chemiluminescence (POCL) reaction detection system. In this study, a microcolumn (100 × 1.0 mm I.D.) was used for separation to obtain higher sensitivity and shorter analysis time.MethodsThe system included automated precolumn ion-exchange extraction of amines, followed by separation on an ODS column, coulometric oxidation, fluorescence derivatization with ethylenediamine, and finally POCL reaction detection.ResultsThe detection limits for CAs and their 3-O-methyl metabolites were 0.3–2.0 fmol. The analysis time was about 35 min, about half that of previously reported results. The method developed was used in monitoring changes in CAs and 3-O-methyl metabolite concentrations in human plasma during exercise.ConclusionThe simultaneous determination method for concentrations of CAs and their 3-O-methyl metabolites in human plasma was developed using micro LC-peroxyoxalate chemiluminescence detection. We were successful in quantitating the changes in plasma CAs and their 3-O-methyl metabolites during exercise.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 366, Issues 1–2, April 2006, Pages 168–173
نویسندگان
, , , , ,