کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1968180 1538761 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Standardization of calibration and quality control using surface enhanced laser desorption ionization-time of flight-mass spectrometry
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Standardization of calibration and quality control using surface enhanced laser desorption ionization-time of flight-mass spectrometry
چکیده انگلیسی

BackgroundProtein profiling by surface enhanced laser desorption ionization-time of flight-mass spectrometry (SELDI-TOF-MS) is gaining importance as a diagnostic tool for a whole range of diseases. This report describes a QC procedure, which acts prospectively by checking the calibration before starting profiling experiments.MethodsA well-defined protocol for calibration of the Protein Biosystem IIc instrument was established, using a commercial QC sample containing independent certified standards and by determination of acceptance criteria. Instrument calibration was performed externally every week with the standards provided by the manufacturer. QC was performed for the period of 5 months.ResultsAccording to the acceptance criteria defined in this study, data points should be in the established range of the process mean ± 2 standard deviations for the mass-to-charge ratios (m / z values), peak intensities, signal-to-noise ratios (S / N), and peak resolutions for insulin and apomyoglobin in the QC sample. Moreover, it was demonstrated that the pipetting variability in the handling of the QC sample significantly contributed to systematic errors and that spotting of a larger volume of QC sample resulted in a better reproducibility.ConclusionsStringent quality control of the calibration part of SELDI-TOF-MS experiments prevents unreliable data acquisition from the very start.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 366, Issues 1–2, April 2006, Pages 249–256
نویسندگان
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