کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1968239 1538762 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The influence of NAT2 genotypes on the plasma concentration of isoniazid and acetylisoniazid in Chinese pulmonary tuberculosis patients
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The influence of NAT2 genotypes on the plasma concentration of isoniazid and acetylisoniazid in Chinese pulmonary tuberculosis patients
چکیده انگلیسی

BackgroundIsoniazid (INH) is widely used in the therapy of tuberculosis. Poor metabolizer (PM) of the NAT2 is an important reason of inter-individual difference of the plasma INH concentration. We studied the relationship between NAT2 genotype and INH and its metabolite acetylisoniazid (AcINH) concentration in Chinese people.MethodForty-six tuberculosis patients were enrolled in the study. Each patient took 300 mg INH daily for at least 7 days. Two hours after the INH was given, the vein blood was drawn. NAT2 genotypes of patients were detected by a reverse dot blot (RDB) method. The plasma concentration of INH and AcINH was determined by a precolumn derivization HPLC method.ResultsIn 46 patients, homozygous mutant (m/m), heterozygous mutant (m/wt) and homozygous wild-type (wt/wt) subjects were 7, 22 and 17, respectively. Plasma concentration of INH and AcINH were 12.74 ± 10.51 and 12.49 ± 9.61 μmol/l, respectively. There was no statistical difference among 3 genotypes. The ratios of AcINH and INH (RA/I) of 3 genotypes were 0.67 ± 0.34, 0.88 ± 0.40 and 1.69 ± 0.66, respectively. The RA/I of m/m and m/wt group were significantly lower than wt/wt group (P < 0.01).ConclusionThe results suggest that various NAT2 genotypes in Chinese tuberculosis patients have great impact on the metabolism capacity of NAT2. This finding maybe valuable in the rational use of relevant drugs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 365, Issues 1–2, March 2006, Pages 104–108
نویسندگان
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