کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1968290 1538764 2006 26 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Present and future of rapid and/or high-throughput methods for nucleic acid testing
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Present and future of rapid and/or high-throughput methods for nucleic acid testing
چکیده انگلیسی

BackgroundBehind the success of ‘completing’ the human genome project was a more than 30-year history of technical innovations for nucleic acid testing.MethodsDiscovery of specific restriction endonucleases and reverse transcriptase was followed shortly by the development of the first diagnostic nucleic acid tests in the early 1970s. Introduction of Southern, Northern and dot blotting and DNA sequencing later in the 1970s considerably advanced the diagnostic capabilities. Nevertheless, it was the discovery of the polymerase chain reaction (PCR) in 1985 that led to an exponential growth in molecular biology and the introduction of practicable nucleic acid tests in the routine laboratory. The past two decades witnessed a continuing explosion of technological innovations in molecular diagnostics. In addition to classic PCR and reverse transcriptase PCR, numerous variations of PCR and alternative amplification techniques along with an ever-increasing variety of detection chemistries, closed tube (homogeneous) assays, and automated systems were developed. Discovery of real-time quantitative PCR and the development of oligonucleotide microarrays, the ‘DNA chip’, in the 1990s heralded the beginning of another revolution in molecular biology and diagnostics that is still in progress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 363, Issues 1–2, January 2006, Pages 6–31
نویسندگان
,