کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1969319 1059765 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stability study of 81 analytes in human whole blood, in serum and in plasma
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Stability study of 81 analytes in human whole blood, in serum and in plasma
چکیده انگلیسی

ObjectiveWe studied the pre-analytical stability of 81 analytes based on the variables of delay before processing, storage as whole blood or serum/plasma, the storage temperature and the type of tube the sample was stored in.Design and methodsThe mean difference between assays for samples from 10 subjects was calculated with the samples being kept under different storage conditions and for different times between sampling time and analysis: up to 24 h for biochemistry, coagulation and hematology, and up to 72 h for hormonology. This difference was compared to the acceptable limits derived from the analytical and the intra individual biological variation.ResultsMost of the analytes investigated remained stable up to 24 h under all storage conditions prior to centrifugation. However, some analytes were significantly affected either by delay, tube type or temperature, such as potassium, inorganic phosphorus, magnesium, LD, glucose, lactate, mean corpuscular volume, mean corpuscular hemoglobin, activated partial thromboplastin time, insulin, C-peptide, PTH, osteocalcin, C-telopeptide and ACTH.ConclusionThis study may be useful to help define acceptable delay times and storage conditions when a short time between sample collection and processing is not possible.


► Pre-analytical stability of 81 analytes was evaluated in whole blood, serum or plasma.
► Changes in concentration during storage before analysis were examined.
► A statistical approach to determine an acceptable total change limit was proposed.
► Influence of temperature, tubes, delay was tested.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Biochemistry - Volume 45, Issue 6, April 2012, Pages 464–469
نویسندگان
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