کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1969500 | 1538893 | 2011 | 6 صفحه PDF | دانلود رایگان |
BackgroundWe have investigated the Capillarys 2 Hemoglobin testing system to assist in presumptive diagnosis of thalassemia and hemoglobinopathies commonly found in Southeast Asia.MethodsStudy was conducted on 226 newborns. Hematological parameters were recorded and Hb profiles were examined on the Capillarys 2 Hemoglobin analyzer (SEBIA). DNA analyses were used to establish the final diagnoses.ResultsAmong 226 newborns examined, 122 had thalassemias with 17 different genotypes. The capillary electrophoresis system could provide useful data for presumptive diagnoses of cases, especially those with Hb E and α-thalassemia. Hb E was found to be 2.6–6.2% in heterozygote whereas Hb Bart's were clearly observed in cases with compound heterozygous or homozygous α+-thalassemia and heterozygous α0-thalassemia. Hb H disease and other forms of α-thalassemia could be differentiated based on the presence of Hb Bart's and its percentage.ConclusionThe capillary electrophoresis system is applicable to newborn screening for common forms of thalassemia in Southeast Asia.
Journal: Clinical Biochemistry - Volume 44, Issues 5–6, April 2011, Pages 406–411