کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1969874 | 1538884 | 2012 | 6 صفحه PDF | دانلود رایگان |
ObjectivesWe developed an ultrasensitive enzyme immunoassay (ICT-EIA) for insulin autoantibody (IAA) measurements to better understand the pathophysiology of diabetes.Design and methodsWe developed ICT-EIA for IAA and measured IAA in 24 patients with type 1 diabetes, 30 patients with type 2 diabetes, 30 patients with methimazole-treated Graves' disease, 20 patients with Hashimoto's disease, 9 patients with hyperinsulinemia, and 73 healthy control subjects.ResultsThe conventional ELISA identified 3 patients with type 1 diabetes and 2 patients with type 2 diabetes as IAA positive, whereas 15 patients with type 1 diabetes, 7 patients with type 2 diabetes, and 4 patients with methimazole-treated Graves' disease were identified as IAA positive using ICT-EIA.ConclusionsThe ICT-EIA is an ultrasensitive and specific assay for IAA, and its use may provide a better understanding of the role of IAA in diabetes onset and progression.
► We developed an ultra-sensitive enzyme immunoassay (ICT-EIA) for IAA measurements.
► The ICT-EIA was more sensitive for the detection of IAA than a conventional ELISA.
► The ICT-EIA will be useful for the early detection and diagnosis of LADA.
Journal: Clinical Biochemistry - Volume 45, Issues 13–14, September 2012, Pages 1086–1091