کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1970014 | 1538900 | 2010 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Are anti-Helicobacter pylori urease antibodies involved in atherosclerotic diseases? Are anti-Helicobacter pylori urease antibodies involved in atherosclerotic diseases?](/preview/png/1970014.png)
ObjectivesThe ureB subunit of urease is a major target recognized by the antibodies of Helicobacter pylori-infected patients. The minimal epitope was determined to be an 8-mer peptide (H-SIKEDVQF-OH).Design and methodsThe aim of this study was to discover whether this synthetic 8-mer peptide (BK-61A) directly recognizes the anti-ureB subunit antibodies of H. pylori-infected and atherosclerotic patients. To achieve a better presentation of the epitopes to antibodies, a new isocyanuric linker was designed and used for to immobilize the peptides on a cellulose support.ResultsIn this study a new peptide synthesis method is presented. Anti-ureB antibodies were evaluated by the dot blot technique in 26 H. pylori-infected donors and the sera of 20 H. pylori-infected patients with atherosclerosis using the 8-mer peptide.ConclusionsThe results reveal that the BK-61A peptide could be used for diagnosing the presence of anti-ureB antibodies that may be involved in the initiation of atherosclerosis.
Journal: Clinical Biochemistry - Volume 43, Issues 1–2, January 2010, Pages 115–123