کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1975353 1060618 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification and properties of malic enzyme from herring Clupea harengus spermatozoa
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Purification and properties of malic enzyme from herring Clupea harengus spermatozoa
چکیده انگلیسی

Herring spermatozoa exhibit higher activity of malic enzyme (ME) than Atlantic salmon (Salmo salar), brown trout (Salmo trutta), carp (Cyprinus carpio) and African catfish (Clarias gariepinus) spermatozoa. Two molecular forms of ME are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme with much lower activity (ratio about 33:1). NAD-preferring ME was purified by chromatography on DEAE-Sepharose, Red Agarose and Sephadex G-200 to a specific activity of 36 μmol/min/mg protein and NADP-specific ME on DEAE-Sepharose and 2′5′-ADP Sepharose. The molecular mass for NAD-preferring and NADP-specific ME determined by SDS-PAGE was equal to 61 and 64 kDa, respectively. High activity of ME suggests adaptation of herring spermatozoa to metabolism at high oxygen tension for herring spawn.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 164, Issue 3, March 2013, Pages 216–220
نویسندگان
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