کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1975861 | 1060659 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bio-Mos®: An effective inducer of dicentracin gene expression in European sea bass (Dicentrarchus labrax)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Bio-Mos®: An effective inducer of dicentracin gene expression in European sea bass (Dicentrarchus labrax) Bio-Mos®: An effective inducer of dicentracin gene expression in European sea bass (Dicentrarchus labrax)](/preview/png/1975861.png)
چکیده انگلیسی
Concern over the use of dietary antibiotics in aquaculture has encouraged the industry to search for alternatives that both enhance performance and afford protection from disease. Bio-Mos®, derived from the outer cell wall of a specific strain of yeast Saccharomyces cerevisiae (Alltech Inc, USA) is a product that fits these criteria. Here, we present data on the impact of a Bio-Mos® supplemented diet on the mRNA copy number of the antimicrobial peptide dicentracin, whose transcript regulation has not yet been explored in fish. We analyzed Bio-Mos®-induced changes in the expression of sea bass (Dicentrarchus labrax) dicentracin, using a one-tube two-temperature real-time RT-PCR with which the gene expression can be absolutely quantified using the standard curve method. Our results revealed that 30Â days of feeding fish with diets containing Bio-Mos® supplemented at either 3â° or 5â° significantly increased the dicentracin mRNA copy number in the head kidney. Furthermore, the mRNA copy number in fish fed at 3â° was significantly higher than that of the group fed at 5â° for the same period of feeding Bio-Mos®. A longer feeding period (60Â days) did not further increase the dicentracin transcript levels as compared to the values recorded after 30Â days of feeding either in the group fed at 3â° or in the one fed at 5â° diet. However, the transcript levels in fish fed at 3â° proved to be significantly higher than those of the controls after 60Â days of feeding. These findings offer new information about the response of antimicrobial peptides at the transcriptional level to diets supplemented with immune response modulators, and support a role of Bio-Mos® in promoting sea bass nonspecific immune system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 153, Issue 4, August 2009, Pages 372-377
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 153, Issue 4, August 2009, Pages 372-377
نویسندگان
Genciana Terova, Andrea Forchino, Simona Rimoldi, Fabio Brambilla, Micaela Antonini, Marco Saroglia,