کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1976357 | 1060685 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of the black bullhead hepatic β-adrenoceptors
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Black bullhead catfish (Ameiurus melas) were exposed to air for 1 h to examine the effect of an acute stress on the distribution and function of the hepatic β-adrenoceptors (β-ARs). Air exposure significantly reduced both adrenaline (ADR)- and noradrenaline (NADR)-stimulated glucose production in isolated hepatocytes with no effect on either receptor affinity (Kd) or number of binding sites (Bmax). A 24 h exposure of isolated hepatocytes to the β-agonist isoproterenol also had no significant impact on either binding parameter. Competition studies using selective agonists and antagonists suggest that the hepatic β-AR in this species is pharmacologically β2-like. However in addition to the β2-AR, molecular evidence provides support for the existence of hepatic β-ARs that phylogenetically group with the β3-ARs and the β1-ARs. Despite the presence of several potential phosphorylation sites in the third intracellular loop and cytoplasmic tail of the bullhead β2-AR, no significant changes were observed in the binding parameters. While physiological data supports the presence of only a single subtype, molecular data supports the existence of multiple β-AR subtypes in this species. The mechanisms thought to regulate mammalian β-ARs exist in the bullhead ARs reported here but these mechanisms are not as effective in this fish system as in mammals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 149, Issue 2, February 2008, Pages 265-274
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 149, Issue 2, February 2008, Pages 265-274
نویسندگان
Stephen G. Dugan, Xi Chen, James G. Nickerson, Colin J. Montpetit, Thomas W. Moon,