کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1976446 1060689 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two glutathione S-transferase isoenzymes purified from Bulinus truncatus (Gastropoda: Planorbidae)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Two glutathione S-transferase isoenzymes purified from Bulinus truncatus (Gastropoda: Planorbidae)
چکیده انگلیسی

We purified and characterized two major glutathione S-transferase isoenzymes (GST2 and GST3) from snail Bulinus truncatus (Mollusca, Gastropoda, Planorbidae) tissue. The Km with respect to 1-chloro-2, 4-dinitrobenzene (CDNB) for both isoenzymes was increased as the pH decreased. Km of both isoenzymes with respect to glutathione (GSH) doubled when the pH was increased from 6.0 to 6.5. Acid inactivated GST2 and GST3 and the two enzymes were almost inactive at pH 3.5. However, they retain the full activity for at least 20 h when incubated at pH between 6.0 and 9.0. The optimum temperature was 45 °C for GST2 and 50 °C for GST3. The half lifetime at 50 °C was 70 min and 45 min for GST2 and GST3 isoenzymes, respectively. Addition of 5 mM GSH to the incubation buffer increased the half life of both isoenzymes more than fourfold. The activation energy for catalyzing the conjugation of CDNB was 1.826 and 3.435 kcal/mol for GST2 and GST3, respectively. I50 values for Cibacron blue, bromosulphophthalein, indocyanine green, hematin and ethacrynic acid were 0.76 μM, 47.9 μM, 7.59 μM, 0.03 μM and 0.79 μM for GST2, and 0.479 μM, 79.4 μM, 89.1 μM, 32.4 μM and 1.15 μM for GST3, respectively. Cibacron blue and indocyanine green were non-competitive inhibitors, while hematin was a mixed inhibitor.Bromosulphophthalein was found to be a competitive inhibitor for GST2 and a mixed inhibitor for GST3.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 143, Issue 1, January 2006, Pages 76–84
نویسندگان
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