Design, synthesis, and characterization of nucleosomes containing site-specific DNA damage

• Mono and dinucleosomes containing single and multiple site-specific DNA damage can now be easily prepared.
• Using specifically positioned DNA sequences the effects of rotational and translational position on DNA damage dynamics and reactivity can be determined.
• Site-specifically damaged nucleosomes have led to a better understanding of the principles governing DNA damage detection and repair in chromatin.

How DNA damaged is formed, recognized, and repaired in chromatin is an area of intense study. To better understand the structure activity relationships of damaged chromatin, mono and dinucleosomes containing site-specific damage have been prepared and studied. This review will focus on the design, synthesis, and characterization of model systems of damaged chromatin for structural, physical, and enzymatic studies.

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