کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1980188 1061828 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization of a putative plant homolog of MBD4 DNA glycosylase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Molecular characterization of a putative plant homolog of MBD4 DNA glycosylase
چکیده انگلیسی


• We have characterized a putative plant homolog of vertebrate MBD4 DNA glycosylase.
• The plant enzyme lacks the distinctive MBD domain of its metazoan counterparts.
• It processes a variety of 5-substituted uracil derivatives when mispaired to guanine.
• After base excision, the enzyme remains bound to the abasic site.
• It shows a preference for a CpG context, where most plant DNA methylation is found.

Methyl-CpG-binding domain 4 (MBD4) DNA glycosylase is involved in excision of spontaneous deamination products of cytosine and 5-methylcytosine in animals, but it is unknown whether related proteins perform similar functions in plants. We report here the isolation and biochemical characterization of a putative MBD4 homolog from Arabidopsis thaliana, designated as MBD4L (MBD4-like). The plant enzyme lacks the MBD domain present in mammalian MBD4 proteins, but conserves a DNA glycosylase domain with critical residues for substrate recognition and catalysis, and it is more closely related to MBD4 homologs than to other members of the HhH-GPD superfamily. Arabidopsis MBD4L excises uracil and thymine opposite G, and the presence of halogen substituents at C5 of the target base greatly increases its excision efficiency. No significant activity is detected on cytosine derivatives such as 5-methylcytosine or 5-hydroxymethylcytosine. The enzyme binds to the abasic site product generated after excision, which decreases its catalytic turnover in vitro. Both the full-length protein and a N-terminal truncated version retaining the catalytic domain exhibit a preference for a CpG sequence context, where most plant DNA methylation is found. Our results suggest that an important function of Arabidopsis MBD4L is to protect the plant genome from the mutagenic consequences of cytosine and 5-methylcytosine deamination.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 12, Issue 11, November 2013, Pages 890–898
نویسندگان
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