کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1980243 | 1061834 | 2013 | 7 صفحه PDF | دانلود رایگان |
Topoisomerase 1 (Top1) resolves transcription-associated supercoils by generating transient single-strand breaks in DNA. Top1 activity in yeast is a major source of transcription-associated mutagenesis, generating a distinctive mutation signature characterized by deletions in short, tandem repeats. A similar signature is associated with the persistence of ribonucleoside monophosphates (rNMPs) in DNA, and it also depends on Top1 activity. There is only partial overlap, however, between Top1-dependent deletion hotspots identified in highly transcribed DNA and those associated with rNMPs, suggesting the existence of both rNMP-dependent and rNMP-independent events. Here, we present genetic studies confirming that there are two distinct types of hotspots. Data suggest a novel model in which rNMP-dependent hotspots are generated by sequential Top1 reactions and are consistent with rNMP-independent hotspots reflecting processing of a trapped Top1 cleavage complex.
► Top1-dependent, rNMP-independent deletions result from a trapped Top1cc.
► Sequential-cleavage mechanism for a Top1- and rNMP-dependent deletions is proposed.
► Transcription and rNMPs have a multiplicative effect on Top1-dependent deletions.
Journal: DNA Repair - Volume 12, Issue 3, 1 March 2013, Pages 205–211