Sensor and effector kinases in DNA damage checkpoint regulate capacity for homologous recombination repair of fission yeast in G2 phase

► Effect of checkpoint-deficiency on DSB repair was studied under forced G2 arrest. ► Repair defect was manifest only after high dose irradiation. ► DSB-induced foci formation by the repair proteins was not affected. ► Disappearance of the foci was markedly delayed. ► The checkpoint has a role downstream of, or in parallel to the core repair reaction.