کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1980380 1061849 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RAD18–BRCTx interaction is required for efficient repair of UV-induced DNA damage
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
RAD18–BRCTx interaction is required for efficient repair of UV-induced DNA damage
چکیده انگلیسی

BRCA1 carboxyl-terminal (BRCT) motifs are present in a number of proteins involved in DNA repair and/or DNA damage signaling pathways. The BRCT domain-containing protein BRCTx has been shown to interact physically with RAD18, an E3 ligase involved in postreplication repair and homologous recombination repair. However, the physiological relevance of the interaction between RAD18 and BRCTx is largely unknown. In this study, we showed that RAD18 interacts with BRCTx in a phosphorylation-dependent manner and that this interaction, mediated via highly conserved serine residues on the RAD18 C terminus, is required for BRCTx accumulation at DNA damage sites. Furthermore, we uncovered critical roles of the RAD18-BRCTx module in UV-induced DNA damage repair but not PCNA mono-ubiquitination or homologous recombination. Thus, our results suggest that RAD18 has an additional function in the surveillance of the UV-induced DNA damage response signal.


► BRCTx is a DNA damage response protein.
► BRCTx interacts with RAD18 in a phosphorylation-dependent manner.
► RAD18–BRCTx interaction is required for UV-induced DNA damage repair.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 11, Issue 2, 1 February 2012, Pages 131–138
نویسندگان
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