کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1980699 | 1061875 | 2008 | 16 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance](/preview/png/1980699.png)
The genes encoding Rev1 and DNA polymerase ζ (Rev3/Rev7) are together required for the vast majority of DNA damage-induced mutations in eukaryotes from yeast to humans. Here, we provide insight into the critical role that the Saccharomyces cerevisiae Rev1 C-terminus plays in the process of mutagenic DNA damage tolerance. The Rev1 C-terminus was previously thought to be poorly conserved and therefore not likely to be important for mediating protein–protein interactions. However, through comprehensive alignments of the Rev1 C-terminus, we have identified novel and hitherto unrecognized conserved motifs that we show play an essential role in REV1-dependent survival and mutagenesis in S. cerevisiae, likely in its post-replicative gap-filling mode. We further show that the minimal C-terminal fragment of Rev1 containing these highly conserved motifs is sufficient to interact with Rev7.
Journal: DNA Repair - Volume 7, Issue 9, 1 September 2008, Pages 1455–1470