کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1980699 1061875 2008 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance
چکیده انگلیسی

The genes encoding Rev1 and DNA polymerase ζ (Rev3/Rev7) are together required for the vast majority of DNA damage-induced mutations in eukaryotes from yeast to humans. Here, we provide insight into the critical role that the Saccharomyces cerevisiae Rev1 C-terminus plays in the process of mutagenic DNA damage tolerance. The Rev1 C-terminus was previously thought to be poorly conserved and therefore not likely to be important for mediating protein–protein interactions. However, through comprehensive alignments of the Rev1 C-terminus, we have identified novel and hitherto unrecognized conserved motifs that we show play an essential role in REV1-dependent survival and mutagenesis in S. cerevisiae, likely in its post-replicative gap-filling mode. We further show that the minimal C-terminal fragment of Rev1 containing these highly conserved motifs is sufficient to interact with Rev7.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 7, Issue 9, 1 September 2008, Pages 1455–1470
نویسندگان
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