کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1981069 | 1061899 | 2007 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Rad52 and Rad59 exhibit both overlapping and distinct functions Rad52 and Rad59 exhibit both overlapping and distinct functions](/preview/png/1981069.png)
Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.
Journal: DNA Repair - Volume 6, Issue 1, 4 January 2007, Pages 27–37