کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1981519 1061950 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of oxidative metabolism on spontaneous Polζ-dependent translesion synthesis in Saccharomyces cerevisiae
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The effect of oxidative metabolism on spontaneous Polζ-dependent translesion synthesis in Saccharomyces cerevisiae
چکیده انگلیسی

DNA lesions can stall or block high-fidelity polymerases, thus inhibiting replication. To bypass such lesions, low-fidelity translesion synthesis (TLS) polymerases can be used to insert a nucleotide across from the lesion or extend from a lesion:base mispair. When DNA repair is compromised in Saccharomyces cerevisiae, spontaneous DNA lesions can lead to a novel mutational event in which a frameshift is accompanied by one or more base pair substitutions. These “complex frameshifts” are dependent upon the TLS polymerase Polζ, and provide a mutational signature for mutagenic Polζ-dependent activity. In the current study, we have found that a specific subset of the Polζ-dependent mutational events requires oxidative metabolism. These results suggest that translesion bypass of spontaneously oxidized DNA bases can be a significant source of mutagenesis in repair compromised cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 5, Issue 2, 3 February 2006, Pages 226–234
نویسندگان
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