کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1981889 1539423 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans
چکیده انگلیسی

Green algae have a great potential as biofactories for the production of proteins. Chlamydomonas reinhardtii, a representative of eukaryotic microalgae, has been extensively used as a model organism to study light-induced gene expression, chloroplast biogenesis, photosynthesis, light perception, cell–cell recognition, and cell cycle control. However, little is known about the glycosylation machinery and N-linked glycan structures of green algae. In this study, we performed mass spectrometry analysis of N-linked oligosaccharides released from total extracts of Chlamydomonas reinhardtii and demonstrated that C. reinhardtii algae possess glycoproteins with mammalian-like sialylated N-linked oligosaccharides. These findings suggest that C. reinhardtii may be an attractive system for expression of target proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Open Bio - Volume 1, December 2011, Pages 15–22
نویسندگان
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