کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1982630 | 1062303 | 2009 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The Pandinus imperator haemolymph lipoprotein, an unusual phosphatidylserine carrying lipoprotein The Pandinus imperator haemolymph lipoprotein, an unusual phosphatidylserine carrying lipoprotein](/preview/png/1982630.png)
The haemolymph lipoprotein of the scorpion, Pandinus imperator was isolated and characterised. Contrary to the lipoproteins of insects and the discoidal HDL-lipoproteins of a crayfish and polychaete, the Pandinus lipoprotein consists of three instead of two apoproteins (apoPiLp I = 230 kDa, apoPiLp II = 130 kDa and apoPiLp III = 120 kDa). The apolipoproteins are arranged in varying stoichiometries as judged by cross-linking experiments. In lipoprotein samples from individual animals, the two smaller subunits occurred in a 1:1 stoichiometry, while the relative amount of the 230 kDa peptide varied. The lipoprotein is a slightly heart-shaped HDL with a diameter of ∼15 nm. It is present in two densities of 1100 and 1190 kg/m3, of which the latter is by far more abundant. The native molecular mass was estimated to be ∼500 kDa. The lipid content was determined as 33.5% and consists of ∼70% neutral lipids and ∼30% phospholipids. Strikingly, 42.5% of the phospholipids is phosphatidylserine while phosphatidylcholine and phosphatidylethanolamine account for 55.1% and ∼2.3%, respectively. Carbohydrate analysis suggests the presence of only high-mannose-type N-glycans. N-glycan profiling shows glycans corresponding to a size of 8.0–11.5 hexose units.
Journal: Insect Biochemistry and Molecular Biology - Volume 39, Issue 10, October 2009, Pages 735–744