کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1982789 | 1062317 | 2008 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification of two genes essential for sperm development in the male tick Amblyomma hebraeum Koch (Acari: Ixodidae) Identification of two genes essential for sperm development in the male tick Amblyomma hebraeum Koch (Acari: Ixodidae)](/preview/png/1982789.png)
In most ticks of the family Ixodidae, gonad maturation and spermatogenesis are stimulated by the taking of a blood meal. Previous work from this laboratory identified 35 genes that are up-regulated by feeding [Weiss, B.L., Stepczynski, J.M., Wong, P., Kaufman, W.R., 2002. Identification and characterization of genes differentially expressed in the testis/vas deferens of the fed male tick, Amblyomma hebraeum. Insect Biochemistry and Molecular Biology 32, 785–793]. The functions of most of these genes remain unknown. We used RNA interference technology to investigate the consequences of blocking the function of 13 of these genes. Attenuation of the expression of two of these in particular, AhT/VD 8 and AhT/VD 10, correlated with deformities in the testis and abnormalities in spermiogenesis. Furthermore, most females fed in the company of these males did not engorge properly and laid many fewer eggs, most of which were infertile.
Journal: Insect Biochemistry and Molecular Biology - Volume 38, Issue 7, July 2008, Pages 721–729