کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1983025 | 1062334 | 2006 | 7 صفحه PDF | دانلود رایگان |
N-ethylmaleimide-sensitive factor (NSF) is a component required for vesicular transport and release of neurotransmitters or neurohormones in the constitutive secretory pathways. Here, the spatial and temporal expression of NSF gene was investigated in Helicoverpa armigera (Helar). Reverse transcription-polymerase chain reaction analysis reveals that Helar-NSF is transcribed preferentially in the nervous system of H. armigera. Using in situ hybridization, the Helar-NSF mRNA is further localized in the superficial layer or deep layer cells of the brain, subesophageal ganglion (SG) and other ganglia. The developmental profiles of Helar-NSF show that both mRNA and protein in pupal brain-SG complexes are significantly higher in nondiapause-destined individuals than in diapause-destined individuals. The expression patterns are consistent with those of two neurohormones, prothoracicotropic hormone (PTTH) and diapause hormone (DH). These data suggest a central function of Helar-NSF in developmental process through regulating neurohormone release.
Journal: Insect Biochemistry and Molecular Biology - Volume 36, Issue 7, July 2006, Pages 603–609