کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1983165 | 1062348 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential usage of two promoters of the Broad-Complex gene in the silkworm, Bombyx mori
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کلمات کلیدی
TSSECRKBPBR-CEcdysone - ادمیزونAlternative splicing - جابجایی جایگزینkilobase pair - جفت کیلوباDifferential transcription - رونویسی دیفرانسیلtranscription start site - رونویسی شروع سایتGenome structure - ساختار ژنومBroad-Complex - مجتمع مسکونیBombyx mori - کرم ابریشمEcdysone receptor - گیرنده اگزایسون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش حشره شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The Broad-Complex gene encodes one of the key regulators of the ecdysone signal cascade. We previously isolated part of the genomic DNA and cDNAs of Broad-Complex in Bombyx mori (BmBR-C). Here, we report structures of the entire genomic DNA and 5â² untranslated region (5â²-UTR) of the cDNAs. BmBR-C was found to span about 158Â kbp including 13 exons. In the 5â²-UTR, additional alternatively spliced exons were identified. The 5â² ends of the cDNAs were mapped to two different positions, the distal promoter (Pdist) and proximal promoter (Pprox), separated by 86Â kbp. Expression from these promoters was controlled differentially. Semi-quantitative PCR using cDNAs from the carcass, silk glands and fat body revealed that expression from Pprox was changed moderately and expression from Pdist was weak and constant during the fourth ecdysis. At the onset of pupation, expression from Pprox was suppressed in all tissues, but that from Pdist was induced in the carcass and ASG. In the fat body, expression from both promoters increased in the prepupal stage. A combination of promoters differing in responsiveness to an ecdysone signal may serve to achieve a complex regulation of downstream genes in reply to a simple hormonal signal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Insect Biochemistry and Molecular Biology - Volume 36, Issue 10, October 2006, Pages 779-788
Journal: Insect Biochemistry and Molecular Biology - Volume 36, Issue 10, October 2006, Pages 779-788
نویسندگان
Yoshinori Nishita, Shigeharu Takiya,