کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1983351 | 1539871 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Global profiling of lysine acetylation in human histoplasmosis pathogen Histoplasma capsulatum
ترجمه فارسی عنوان
پروفیل جهانی استیلاسیون لیزین در پاتوژن هیستوپلاسما انسان هیستوپلاسما کپسولاتوم
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کلمات کلیدی
استریل لیزین، طیف سنجی جرمی، هیستوپلاسما کپسولاتوم، سرگیجه،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Histoplasma capsulatum is the causative agent of human histoplasmosis, which can cause respiratory and systemic mycosis in immune-compromised individuals. Lysine acetylation, a protein posttranslational protein modification, is widespread in both eukaryotes and prokaryotes. Although increasing evidence suggests that lysine acetylation may play critical roles in fungus physiology, very little is known about its extent and function in H. capsulatum. To comprehensively profile protein lysine acetylation in H. capsulatum, we performed a global acetylome analysis through peptide prefractionation, antibody enrichment, and LCâMS/MS analysis, identifying 775 acetylation sites on 456 acetylated proteins; and functionally analysis showing their involvement in different biological processes. We defined six types of acetylation site motifs, and the results imply that lysine residue of polypeptide with tyrosine at the â1 and +1 positions, histidine at the +1 position, and phenylalanine (F) at the +1 and +2 position is a preferred substrate of lysine acetyltransferase. Moreover, some virulence factors candidates including calmodulin and DnaK are acetylated. In conclusion, our data set may serve as an important resource for the elucidation of associations between functional protein lysine acetylation and virulence in H. capsulatum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 73, April 2016, Pages 1-10
Journal: The International Journal of Biochemistry & Cell Biology - Volume 73, April 2016, Pages 1-10
نویسندگان
Longxiang Xie, Wenjie Fang, Wanyan Deng, Zhaoxiao Yu, Juan Li, Min Chen, Wanqing Liao, Jianping Xie, Weihua Pan,