کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1983790 | 1539905 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle](/preview/png/1983790.png)
چکیده انگلیسی
The role of polyamines at the G1/S boundary and in the G2/M phase of the cell cycle was studied using synchronized HeLa cells treated with thymidine or with thymidine and aphidicolin. Synchronized cells were cultured in the absence or presence of α-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase, plus ethylglyoxal bis(guanylhydrazone) (EGBG), an inhibitor of S-adenosylmethionine decarboxylase. When polyamine content was reduced by treatment with DFMO and EGBG, the transition from G1 to S phase was delayed. In parallel, the level of p27Kip1 was greatly increased, so its mechanism was studied in detail. Synthesis of p27Kip1 was stimulated at the level of translation by a decrease in polyamine levels, because of the existence of long 5â²-untranslated region (5â²-UTR) in p27Kip1 mRNA. Similarly, the transition from the G2/M to the G1 phase was delayed by a reduction in polyamine levels. In parallel, the number of multinucleate cells increased by 3-fold. This was parallel with the inhibition of cytokinesis due to an unusual distribution of actin and α-tubulin at the M phase. Since an association of polyamines with chromosomes was not observed by immunofluorescence microscopy at the M phase, polyamines may have only a minor role in structural changes of chromosomes at the M phase. In general, the involvement of polyamines at the G2/M phase was smaller than that at the G1/S boundary.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 6, June 2013, Pages 1042-1050
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 6, June 2013, Pages 1042-1050
نویسندگان
Tomoko Yamashita, Kazuhiro Nishimura, Ryotaro Saiki, Hiroyuki Okudaira, Mayuko Tome, Kyohei Higashi, Mizuho Nakamura, Yusuke Terui, Kunio Fujiwara, Keiko Kashiwagi, Kazuei Igarashi,