کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1984847 | 1539988 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and identification of mouse Cklfsf2a and Cklfsf2b, two homologues of human CKLFSF2
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کلمات کلیدی
TM4SFMGDMMTVCKLFRT-PCRGAPDHORFLUCchemokine-like factorEST - استMale reproduction - تولید مثل مردExpressed Sequence Tag - تکرار اظهار نظرreverse transcription PCR - رونویسی معکوس PCRopen-reading frame - قاب خواندن بازluciferase - لوسیفرازMARVEL - مارولUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهHomologue - همولوگpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازMouse mammary tumor virus - ویروس تومور پستان موشglyceraldehydes-3-phosphate dehydrogenase - گلیسرالید هیدروژن 3-فسفات دهیدروژنازAndrogen Receptor - گیرنده آندروژنی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular cloning and identification of mouse Cklfsf2a and Cklfsf2b, two homologues of human CKLFSF2 Molecular cloning and identification of mouse Cklfsf2a and Cklfsf2b, two homologues of human CKLFSF2](/preview/png/1984847.png)
چکیده انگلیسی
Human chemokine-like factor superfamily (CKLFSF) is a novel gene family comprising CKLF and CKLFSF1-8. Among them, CKLFSF2 is highly expressed in testis and may play important roles in male reproduction. Besides, it is very active during evolution and has two counterparts in mouse. For further study, we cloned the two mouse genes by EST assembly and RT-PCR methods and designated them as mouse Cklfsf2a and Cklfsf2b. Their predicted open-reading frames (ORFs) that encode 169 and 210 amino acids, respectively, were obtained; and their predicted full-length molecular sizes that are approximately 1.2Â kb for mCklfsf2a and 0.9Â kb for mCklfsf2b were confirmed by Northern blot analysis. Mouse Cklfsf2a and Cklfsf2b show similarities with human CKLFSF2 in the expression patterns that are abundant in testis, hematopoietic and immune tissues; as well as in the chromosome localizations that neighbor CKLFSF1 and 3. Their putative protein products have 47.6 and 45.5% identities with hCKLFSF2, respectively; both of them contain four potential transmembrane regions and MARVEL domains, which are also similar with hCKLFSF2. Functionally, they all can affect the transcriptional activity of androgen receptor in PC-3 and HeLa cells, but mCklfsf2a is a repressor while mCklfsf2b and hCKLFSF2 are enhancers. Taken together, we conclude that mouse Cklfsf2a and Cklfsf2b are two homologues of human CKLFSF2. Studies on them would provide much help in further investigation of the latter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 38, Issue 3, March 2006, Pages 420-429
Journal: The International Journal of Biochemistry & Cell Biology - Volume 38, Issue 3, March 2006, Pages 420-429
نویسندگان
Ting Li, Wenling Han, Tian Yang, Peiguo Ding, Min Rui, Dazhen Liu, Ying Wang, Dalong Ma,