کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1984934 | 1539951 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for aerobic ATP synthesis in isolated myelin vesicles
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کلمات کلیدی
PBSCLSMMitoTrackerMBPANTAK3ToMMTPIMVTIMATP synthase - ATP سنتازrelative optical density - تراکم نسبی نسبیOxygen absorption - جذب اکسیژنRh-123 - ر-123Rhodamine 123 - رودامین 123Phosphate buffered saline - فسفات بافر شورRespiratory complexes - مجتمع های تنفسیMitochondrial transition pore - منافذ گذرا میتوکندریاییMyelin - میلینconfocal laser scanning microscopy - میکروسکوپهای اسکن لیزری کانفوکالNeuroenergetics - نوروفناوریMolecular weight - وزن مولکولیWestern blot - وسترن بلاتMyelin basic protein - پروتئین پایه میلینGlutaraldehyde - گلوتارالدئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Even though brain represents only 2-3% of the body weight, it consumes 20% of total body oxygen, and 25% of total body glucose. This sounds surprising, in that mitochondrial density in brain is low, while mitochondria are thought to be the sole site of aerobic energy supply. These data would suggest that structures other than mitochondria are involved in aerobic ATP production. Considering that a sustained aerobic metabolism needs a great surface extension and that the oxygen solubility is higher in neutral lipids, we have focused our attention on myelin sheath, the multilayered membrane produced by oligodendrocytes, hypothesizing it to be an ATP production site. Myelin has long been supposed to augment the speed of conduction, however, there is growing evidence that it exerts an as yet unexplained neuro-trophic role. In this work, by biochemical assays, Western Blot analysis, confocal laser microscopy, we present evidence that isolated myelin vesicles (IMV) are able to consume O2 and produce ATP through the operation of a proton gradient across their membranes. Living optic nerve sections were exposed to MitoTracker, a classical mitochondrial dye, by a technique that we have developed and it was found that structures closely resembling nerve axons were stained. By immunohystochemistry we show that ATP synthase and myelin basic protein colocalize on both IMV and optic nerves. The complex of data suggests that myelin sheath may be the site of oxygen absorption and aerobic metabolism for the axons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 41, Issue 7, July 2009, Pages 1581-1591
Journal: The International Journal of Biochemistry & Cell Biology - Volume 41, Issue 7, July 2009, Pages 1581-1591
نویسندگان
Silvia Ravera, Isabella Panfoli, Daniela Calzia, Maria Grazia Aluigi, Paolo Bianchini, Alberto Diaspro, Gianluigi Mancardi, Alessandro Morelli,