کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1988201 | 1540314 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phospholipase D from Allium sativum bulbs: A highly active and thermal stable enzyme
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کلمات کلیدی
PLDGDHBCAAllium sativumPIP2bicinchoninic acid - بیسینکنینیک اسیدStability - ثباتphosphatidylcholine - فسفاتیدیل کولینphosphatidylethanol - فسفاتیدیلنتانولphosphatidylinositol-4,5-bisphosphate - فسفاتیدیلینستول-4،5-بیسفسفاتPhospholipase D - فسفولیپاز Dactivity - فعالیتglutamate dehydrogenase - گلوتامات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Phospholipase D from Allium sativum bulbs: A highly active and thermal stable enzyme Phospholipase D from Allium sativum bulbs: A highly active and thermal stable enzyme](/preview/png/1988201.png)
چکیده انگلیسی
This is the first report on the identification and partial characterization of phospholipase D (EC 3.1.4.4) from Allium sativum (garlic) bulbs (PLDGB). The enzyme shares the phenomenon of interfacial activation with other lipolytic enzymes, i.e. the hydrolytic rate increases when the substrate changes to a more aggregated state. The enzyme activity is highly temperature tolerant and the temperature optimum was measured to be 70 °C. PLDGB unlike many plant PLDs exhibited high thermal stability. It was activated further after exposure to high temperatures, i.e. 80 °C, indicating that the enzyme refolds better upon cooling back to room temperature after short exposure to thermal stress. The activity of PLDGB is optimum in 70 mM calcium ion concentration and the enzyme is activated further in the presence of phosphatidyl-4,5-bisphosphate (PIP2). PLDGB exhibited both hydrolytic and transphosphatidylation activities, both of which appear to be higher than those of PLD from cabbage leaves (PLDCL).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 42, Issue 4, 1 May 2008, Pages 380-385
Journal: International Journal of Biological Macromolecules - Volume 42, Issue 4, 1 May 2008, Pages 380-385
نویسندگان
Hafeeza Khatoon, Sariya Talat, Hina Younus,