کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1988226 1540317 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Irreversible thermal denaturation of Trichoderma reesei endo-1,4-β-xylanase II and its three disulfide mutants characterized by differential scanning calorimetry
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Irreversible thermal denaturation of Trichoderma reesei endo-1,4-β-xylanase II and its three disulfide mutants characterized by differential scanning calorimetry
چکیده انگلیسی

Kinetic as well as energetic aspects of the thermal denaturation of Trichoderma reesei endo-1,4-β-xylanase II (TRX II) and its three thermostable disulfide mutants were characterized by means of differential scanning calorimetry (DSC) in different solution conditions. The calorimetric transitions were strongly scan-rate dependent, characteristic for an irreversible, kinetically controlled protein denaturation. The DSC-determined T*-values (the temperature at which the denaturation rate constant equals 1 min−1), and the activation free energies for the transitions are consistent with the apparent transition temperatures of TRX II determined earlier by mass spectrometry. Protein aggregation, connected with the irreversibility of the transitions, was present in all cases but was less pronounced with the mutants as well as highly dependent on experimental conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 42, Issue 1, 1 January 2008, Pages 75–80
نویسندگان
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