کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1992288 | 1541077 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of protein kinase C in aldosterone-induced non-genomic inhibition of basolateral potassium channels in human colonic crypts
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Role of protein kinase C in aldosterone-induced non-genomic inhibition of basolateral potassium channels in human colonic crypts Role of protein kinase C in aldosterone-induced non-genomic inhibition of basolateral potassium channels in human colonic crypts](/preview/png/1992288.png)
چکیده انگلیسی
Aldosterone produces rapid, non-genomic, inhibition of basolateral intermediate conductance K+ (IKCa) channels in human colonic crypt cells but the intracellular second messengers involved are unclear. We therefore evaluated the role of protein kinase C (PKC) in aldosterone's non-genomic inhibitory effect on basolateral IKCa channels in crypt cells from normal human sigmoid colon. Patch clamp studies revealed that in cell-attached patches, IKCa channel activity decreased progressively to 38 ± 8% (P < 0.001) of the basal value 10 min after the addition of 1 nmol/L aldosterone, and decreased further to 23 ± 6% (P < 0.02) of the basal value 5 min after increasing the aldosterone concentration to 10 nmol/L. Pre-incubation of crypts with 1 μmol/L chelerythrine chloride or 1 μmol/L Gö 6976 (PKC inhibitors) prevented the inhibitory effect of aldosterone. Conversely, channel activity decreased to 60 ± 9% (P < 0.02) of the basal value 10 min after the addition of 500 nmol/L PMA (a PKC activator), whereas 4α-PMA (an inactive ester) had no effect. When aldosterone (10 nmol/L) and PMA were added together, IKCa channel activity was inhibited to the same extent as with aldosterone alone. These results indicate that aldosterone's non-genomic inhibitory effect on the macroscopic basolateral K+ conductance in human colonic crypts reflects PKC-mediated inhibition of IKCa channels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 104, Issues 1â2, April 2007, Pages 45-52
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 104, Issues 1â2, April 2007, Pages 45-52
نویسندگان
Kate A. Bowley, John E. Linley, Gerrard G. Robins, Shashikala Kopanati, Malcolm Hunter, Geoffrey I. Sandle,