کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1992634 | 1541088 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptional regulation of the rainbow trout CYP19a gene by FTZ-F1 homologue
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
In rainbow trout, there are at least two CYP19 genes (CYP19a and CYP19b). They encode distinct P450arom isozymes that are differentially expressed in the ovary and brain. To understand the transcriptional regulation of the rainbow trout CYP19a (rtCYP19a) gene in the ovary, we isolated its 5â²-flanking region. The presence of potential FTZ-F1-binding sites prompted us to isolate the cDNA encoding a rainbow trout FTZ-F1 homologue (rtFTZ-F1) and analyze its effect on the rtCYP19a gene transcriptional activity. RT-PCR analysis showed overlapping expression of the rtCYP19a and rtFTZ-F1 genes in the ovary. Transient transfection studies in Chinese hamster ovary-derived CHO-K1 cells revealed that the region from â247 to â105, which contains three potential FTZ-F1-binding sites, was required for rtFTZ-F1-mediated transcriptional activation of the rtCYP19a gene. Among the three potential binding sites, the two from â150 to â142 and from â118 to â110 showed strong affinities for rtFTZ-F1 in gel shift assays, and base substitutions in either site almost abolished the transcriptional activation by rtFTZ-F1. Taken together, these results demonstrate that rtFTZ-F1 plays an important role in the transcriptional regulation of the rtCYP19a gene in the ovary.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 99, Issues 2â3, May 2006, Pages 85-92
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 99, Issues 2â3, May 2006, Pages 85-92
نویسندگان
Hiromi Kanda, Takayuki Okubo, Nobuhiro Omori, Hiroko Niihara, Naoko Matsumoto, Kayo Yamada, Shin Yoshimoto, Michihiko Ito, Shinya Yamashita, Tadayoshi Shiba, Nobuhiko Takamatsu,