Applying antibiotic selection markers for nematode genetics

• Antibiotic selection is a cost effective method for nematode transgenesis.
• We provide a simple microinjection protocol for antibiotic resistant transformation.
• Antibiotic marker advantages can apply to many nematode genetic approaches.

Antibiotic selection markers have been recently developed in the multicellular model organism Caenorhabditis elegans and other related nematode species, opening great opportunities in the field of nematode transgenesis. Here we describe how these antibiotic selection systems can be easily combined with many well-established genetic approaches to study gene function, improving time- and cost-effectiveness of the nematode genetic toolbox.