کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1993857 | 1064713 | 2012 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioinformatic analysis of barcoded cDNA libraries for small RNA profiling by next-generation sequencing
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
The characterization of post-transcriptional gene regulation by small regulatory RNAs of 20–30 nt length, particularly miRNAs and piRNAs, has become a major focus of research in recent years. A prerequisite for the characterization of small RNAs is their identification and quantification across different developmental stages, normal and diseased tissues, as well as model cell lines. Here we present a step-by-step protocol for the bioinformatic analysis of barcoded cDNA libraries for small RNA profiling generated by Illumina sequencing, thereby facilitating miRNA and other small RNA profiling of large sample collections.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 58, Issue 2, October 2012, Pages 171–187
Journal: Methods - Volume 58, Issue 2, October 2012, Pages 171–187
نویسندگان
Thalia A. Farazi, Miguel Brown, Pavel Morozov, Jelle J. ten Hoeve, Iddo Z. Ben-Dov, Volker Hovestadt, Markus Hafner, Neil Renwick, Aleksandra Mihailović, Lodewyk F.A. Wessels, Thomas Tuschl,