کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993942 1064720 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Visualizing interaction of proteins relevant to Alzheimer's disease in intact cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Visualizing interaction of proteins relevant to Alzheimer's disease in intact cells
چکیده انگلیسی
To understand normal function of memory studying models of pathological memory decline is essential. The most common form of dementia leading to memory decline is Alzheimer's disease (AD), which is characterized by the presence of neurofibrillary tangles and amyloid plaques in the affected brain regions. Altered production of amyloid β (Aβ) through sequential cleavage of amyloid precursor protein (APP) by β- and γ-secretases seems to be a central event in the molecular pathogenesis of the disease. Thus, the study of the complex interplay of proteins that are involved in or modify Aβ production is very important to gain insight into the pathogenesis of AD. Here, we describe the use of Fluorescence lifetime imaging microscopy (FLIM), a Fluorescence resonance energy transfer (FRET)-based method, to visualize protein-protein-interaction in intact cells, which has proven to be a valuable method in AD research.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 44, Issue 4, April 2008, Pages 299-303
نویسندگان
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