کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994160 1064744 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae
چکیده انگلیسی

How DNA repair proteins interact with the dynamic structure of chromatin is an emerging question. Chromatin structure impedes the access of repair proteins to sites of DNA damage. Several recent studies have implicated chromatin remodeling complexes in DNA repair. In this report we summarize the methods we used to investigate chromatin remodeling during nucleotide excision repair (NER) in vivo. We describe a procedure to analyze UV-induced chromatin remodeling at the silent mating-type locus HML using isolated nuclei from UV-treated yeast cells. In addition, a method to capture transient protein–protein associations in chromatin is outlined. We have used the methods described here to demonstrate that the SWI/SNF chromatin remodeling complex is involved in chromatin rearrangement during NER.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 48, Issue 1, May 2009, Pages 19–22
نویسندگان
, , , ,