کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1994398 | 1064779 | 2007 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Analysis of cell cycle phases and progression in cultured mammalian cells Analysis of cell cycle phases and progression in cultured mammalian cells](/preview/png/1994398.png)
Fluorescence activated cell sorting (FACS) analysis has become a standard tool to analyze cell cycle distributions in populations of cells. These methods require relatively large numbers of cells, and do not provide optimal resolution of the transitions between cell cycle phases. In this report we describe in detail complementary methods that utilize the incorporation of nucleotide analogs combined with microscopic examination. While often more time consuming, these protocols typically require far fewer cells, and allow accurate kinetic assessment of cell cycle progression. We also describe the use of a technique for the synchronization of adherent cells in mitosis by simple mechanical agitation (mitotic shake-off) that eliminates physiological perturbation associated with drug treatments.
Journal: Methods - Volume 41, Issue 2, February 2007, Pages 143–150